Immunological Sciences

Caspase 3 (cleaved) p17 (D175) Rabbit Polyclonal Antibody

Western blot analysis of lysates from 293 cells, treated with Etoposide 25uM 60', using Caspase 3 (Cleaved-Asp175) Antibody. The lane on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from 293 cells, treated with Etoposide 25uM 60', using Caspase 3 (Cleaved-Asp175) Antibody. The lane on the right is blocked with the synthesized peptide.

Catalog Number:AB-83773
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:Poly
Immunogen:The antiserum was produced against synthesized peptide derived from human Caspase 3. AA range:126- 175
Reactivity:HU, Ms,Rt
Applications:Western Blot: 1:500-2000 Immunohistochemistry (paraffin embedded tissues) :  1:50-300 Immunofluorescence:   1:50-300
Molecular Weight:17kDA cleaved & 34kDA non cleaved
Purification:The antibody was affinity- purified from rabbit antiserum by affinity- chromatography using epitope- specific immunogen.
Form:Liquid
Synonyms:CASP3; CPP32; Caspase-3; CASP-3; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; SCA- 1

Applications

Western Blot: 1:500-2000 Immunohistochemistry (paraffin embedded tissues) :  1:50-300 Immunofluorescence:   1:50-300

Immunogen

The antiserum was produced against synthesized peptide derived from human Caspase 3. AA range:126- 175

Target Background

caspase 3(CASP3) Homo sapiens This gene encodes a protein which is a member of the cysteine- aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.

Synonyms:CASP3; CPP32; Caspase-3; CASP-3; Apopain; Cysteine protease CPP32; CPP-32; Protein Yama; SREBP cleavage activity 1; SCA- 1

Storage

Store at -20°C. Avoid repeated freeze-thaw cycles.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Western blot analysis of lysates from 293 cells, treated with Etoposide 25uM 60', using Caspase 3 (Cleaved-Asp175) Antibody. The lane on the right is blocked with the synthesized peptide.

Immunofluorescence analysis of Human-lung-cancer tissue. 1,Cleaved-Caspase-3 p17 (D175) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunohistochemical analysis of paraffin-embedded Human-colon tissue. 1,Cleaved-Caspase-3 p17 (D175) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

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