Catenin gamma /CTNNG1

Western blot analysis of extracts of various cell lines, using γ-Catenin antibody at 1:500   dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L)  at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.  Detection: ECL West Pico Plus.

Western blot analysis of extracts of various cell lines, using γ-Catenin antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus.

Catalog Number:AB-90215
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human γ-Catenin.
Reactivity:Hu, Ms, Rt
Applications:Western Blot: 1:500 - 1:2000 Immunofluorescence: 1:50 - 1:200 Immunoprecipitation: 1:50 - 1:100
Molecular Weight:82kDa
Purification:Aff. Pur
Form:Liquid
Synonyms:JUP;ARVD12;CTNNG;DP3;DPIII;PDGB;PKGB

Applications

Western Blot: 1:500 - 1:2000 Immunofluorescence: 1:50 - 1:200 Immunoprecipitation: 1:50 - 1:100

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human γ-Catenin.

Target Background

This gene encodes a major cytoplasmic protein which is the only known constituent common to submembranous plaques of both desmosomes and intermediate junctions. This protein forms distinct complexes with cadherins and desmosomal cadherins and is a member of the catenin family since it contains a distinct repeating amino acid motif called the armadillo repeat. Mutation in this gene has been associated with Naxos disease. Alternative splicing occurs in this gene; however, not all transcripts have been fully described.

Synonyms:JUP;ARVD12;CTNNG;DP3;DPIII;PDGB;PKGB

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.02% sodium azide,50% glycerol,pH7.3.

Western blot analysis of extracts of various cell lines, using γ-Catenin antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus.

Immunoprecipitation analysis of 200ug extracts of MCF-7 cells, using 3 ug γ-Catenin antibody. Western blot was performed from the immunoprecipitate using γ-Catenin antibody at a dilition of 1:1000.

Immunofluorescence analysis of HeLa cells using γ-Catenin antibody. Blue: DAPI for nuclear staining.

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