Immunological Sciences

CD117/c-kit

Western blot analysis of lysates from COLO205 cells, using CD117/c-kit Antibody. The lane on the right is blocked with the synthesized peptide

Western blot analysis of lysates from COLO205 cells, using CD117/c-kit Antibody. The lane on the right is blocked with the synthesized peptide

Catalog Number:AB-81853
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:Synthesized peptide derived from human c-Kit. AA range: 906-955
Reactivity:Hu, Ms, Rt
Applications:Western Blot: 1:500 – 1:2000. IHC-P: 1:100-1:300 IF: 1:100-1:300 ELISA: 1:20000.
Molecular Weight:117 kDa
Purification:Aff. Pur.
Form:Liquid

Applications

Western Blot: 1:500 – 1:2000. IHC-P: 1:100-1:300 IF: 1:100-1:300 ELISA: 1:20000.

Immunogen

Synthesized peptide derived from human c-Kit. AA range: 906-955

Target Background

This gene encodes the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. This protein is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene.

Storage

Store at -20℃. Avoid freeze / thaw cycles

Buffer

PBS with 0.02% sodium azide, 50% glycerol, 0.5% BSA

Western blot analysis of lysates from COLO205 cells, using CD117/c-kit Antibody. The lane on the right is blocked with the synthesized peptide

Immunohistochemical analysis of paraffin-embedded Human Mammary cancer. 1.  Antibody was diluted at 1:400 (4°overnight). 2.  High-pressure and temperature EDTA,pH8.0 was used for antigen retrieval. 3.  Secondary antibody was diluted at 1:200(RT, 30min)

Immunofluorescence analysis of Hela cell. 1.  c-Kit Polyclonal Antibody (green) wasdiluted at 1:200 (4°overnight). 2.  Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000 (room temperature,50min). 3.  DAPI (blue) 10min

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