Immunological Sciences

CD3 epsilon (activation epitope) Mouse Monoclonal Antibody

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Catalog Number:MAB-94775
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Mouse
Isotype:IgG1
Clone:APA1/1
Immunogen:Purified human CD3 proteins isolated from thymus
Reactivity:Mouse, Human
Applications:Flow cytometry: 1-4 μg/ml; positive control: human T cells stimulated with anti-CD3 (MEM-57) antibody (1 μg/ml). Immunocytochemistry: Fixed and permeabilised cells. The antibody can distinguish TCR-stimulated from non-stimulated cells. Immunoprecipitation Western blot Immunohistochemistry (frozen tissues)
Purification:Purified by protein-A affinity chromatography.
Form:Liquid
Synonyms:CD3 epsilon chain, T3E, IMD18

Applications

Flow cytometry: 1-4 μg/ml; positive control: human T cells stimulated with anti-CD3 (MEM-57) antibody (1 μg/ml). Immunocytochemistry: Fixed and permeabilised cells. The antibody can distinguish TCR-stimulated from non-stimulated cells. Immunoprecipitation Western blot Immunohistochemistry (frozen tissues)

Immunogen

Purified human CD3 proteins isolated from thymus

Target Background

Sample preparation: At the end of stimulation of T cells, perform staining of surface markers (if required) in PBS + 0.1% BSA for 20 min. on ice. Wash with PBS and fix with 2% formaldehyde, 30 min on ice. Wash with PBS and incubate in PBS + 0,1% saponine, 5 min. RT. Incubate the cells in PBS + 1% BSA + 0.03% saponine, 15 min. on ice. Incubate with fluorescence-labeled APA1/1 antibody (1-2 μg/ml) in PBS + 1% BSA + 0.03% saponine in dark, 20 min. RT. Wash with PBS + 1% BSA + 0.03% saponine, resuspend in PBS.

Synonyms:CD3 epsilon chain, T3E, IMD18

Storage

Store at 2-8°C. Do not freeze.

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

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