CD31 / PECAM1 Rabbit Polyclonal Antibody

Western blot analysis of extracts of  various cell lines, using CD31
antibody at 1:1000 dilution.  Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000
dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.    
Exposure time: 3s.

Western blot analysis of extracts of various cell lines, using CD31 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 3s.

Catalog Number:AB-83615
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 600 to the C-terminus of mouse CD31
Reactivity:Hu,Ms,Rt
Applications:Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:200 Immunoprecipitation: 1:50 - 1:200 Immunofluorescence: 1:50-1:200
Molecular Weight:130kDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:PECAM1;CD31;CD31/EndoCAM;GPIIA;PECA1;PECAM-1;e ndoCAM

Applications

Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:200 Immunoprecipitation: 1:50 - 1:200 Immunofluorescence: 1:50-1:200

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 600 to the C-terminus of mouse CD31

Target Background

The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation.

Synonyms:PECAM1;CD31;CD31/EndoCAM;GPIIA;PECA1;PECAM-1;e ndoCAM

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.02% sodium azide,50% glycerol,pH7.3.

Western blot analysis of extracts of various cell lines, using CD31 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 3s.

Immunohistochemistry of paraffin embedded human esophagus using CD31 antibody at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HUVEC cells using CD31 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U2OS cells using CD31 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Review for CD31 Rabbit Polyclonal Antibody Experiment Type: Immunofluorescence (IF) Sample: Rat lung

Review for CD31 Rabbit Polyclonal Antibody Experiment Type: Immunohistochemistry(IHC) Sample: Human prostate Description: Immunohistochemistry analysis of Human prostate, using PECAM1 Polyclonal Antibody.

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