CD31/PECAM1 Rabbit Monoclonal Antibody
Western blot analysis of various lysates, using CD31/PECAM1 Rabbit mAb at 1:12500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L)at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit Negative control (NC): SK-BR-3 Exposure time: 180s.
| Catalog Number: | MAB19014 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Clone: | F2N3M |
| Immunogen: | Recombinant protein.This information is considered to be commercially sensitive. |
| Reactivity: | Human,Mouse,Rat |
| Applications: | WB 1:5000 - 1:13000 IHC-P 1:500 - 1:3000 IF/ICC 1:200 - 1:600 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements. |
| Molecular Weight: | 135kDa |
| Purification: | Affinity purification |
| Form: | liquid |
| Synonyms: | CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM; CD31/PECAM1 |
The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation.
Applications
WB 1:5000 - 1:13000 IHC-P 1:500 - 1:3000 IF/ICC 1:200 - 1:600 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Immunogen
Recombinant protein.This information is considered to be commercially sensitive.
