CD31 Rabbit Polyclonal Antibody

Western blot analysis of CD31 in HepG2, K562, L929, mouse brain lysates using CD31 antibody.

Western blot analysis of CD31 in HepG2, K562, L929, mouse brain lysates using CD31 antibody.

Catalog Number:ABN03853
Conjugate:Unconjugated
Size:100μL
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:The antiserum was produced against synthesized peptide derived from human PECAM-1. AA range:686-735
Reactivity:Human,Mouse
Applications:WB 1:500-1:1000,IHC 1:50-1:100,ICC/IF 1:50-1:200,ELISA 1:5000-1:20000
Molecular Weight:83 kDa
Purification:Affinity purification
Form:liquid
Synonyms:Platelet endothelial cell adhesion molecule; PECAM-1; EndoCAM; GPIIA'; PECA1; CD antigen CD31

PECAM-1 a cell adhesion molecule expressed on platelets and at endothelial cell intercellular junctions. Plays a role in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, cell migration, negative regulation of immune cell signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. Undergoes alternative splicing, generating multiple isoforms in vascular beds of various tissues.

Applications

WB 1:500-1:1000,IHC 1:50-1:100,ICC/IF 1:50-1:200,ELISA 1:5000-1:20000

Immunogen

The antiserum was produced against synthesized peptide derived from human PECAM-1. AA range:686-735

Target Background

PECAM-1 a cell adhesion molecule expressed on platelets and at endothelial cell intercellular junctions. Plays a role in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, cell migration, negative regulation of immune cell signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. Undergoes alternative splicing, generating multiple isoforms in vascular beds of various tissues.

Synonyms:Platelet endothelial cell adhesion molecule; PECAM-1; EndoCAM; GPIIA'; PECA1; CD antigen CD31

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% protective protein and 0.02% sodium azide, pH 7.3.

Western blot analysis of CD31 in HepG2, K562, L929, mouse brain lysates using CD31 antibody.

Immunofluorescence analysis of CD31 in HeLa using CD31 antibody. The picture on the right is blocked using synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded CD31 in paraffin-embedded Human kidney using CD31 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

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