Immunological Sciences

CD366 / TIM-3/ (HAVCR2)

Western blot analysis of extracts of various cell lines, using TIM-3/HAVCR2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Bas Exposure time: 10s.

Western blot analysis of extracts of various cell lines, using TIM-3/HAVCR2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Bas Exposure time: 10s.

Catalog Number:AB-84287
Size:100ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 22-202 of human TIM-3/HAVCR2
Reactivity:Hu, Ms, Rt
Applications:Western Blot: 1:500 - 1:1000 Immunohistochemistry: 1:50 - 1:200
Molecular Weight:33kDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:HAVCR2;CD366;HAVcr-2;KIM-3;TIM3;TIMD-3;TIMD3;T im-3

Applications

Western Blot: 1:500 - 1:1000 Immunohistochemistry: 1:50 - 1:200

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 22-202 of human TIM-3/HAVCR2

Target Background

The protein encoded by this gene belongs to the immunoglobulin superfamily, and TIM family of proteins. CD4-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas, Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. This protein is a Th1-specific cell surface protein that regulates macrophage activation, and inhibits Th1-mediated auto- and alloimmune responses, and promotes immunological tolerance

Synonyms:HAVCR2;CD366;HAVcr-2;KIM-3;TIM3;TIMD-3;TIMD3;T im-3

Storage

Store at -20°C, and avoid repeat freeze-thaw cycles.

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3

Western blot analysis of extracts of various cell lines, using TIM-3/HAVCR2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Bas Exposure time: 10s.

Western blot analysis of extracts of various cell lines, using TIM-3/HAVCR2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 30s.

Immunohistochemistry of Paraffin embedded human tonsil using TIM-3/HAVCR2 antibody At dilution of 1:200 (40x lens).

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