Immunological Sciences

CD81

Western blot analysis of lysate from 4T1 cells, using CD81 Antibody.

Western blot analysis of lysate from 4T1 cells, using CD81 Antibody.

Catalog Number:AB-83878
Size:100μg
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:Synthesized peptide derived from human CD81
Reactivity:Ms
Applications:Western Blot : 1:500-1:2000 Immunofluorescence: 1:50-200
Molecular Weight:23kDa
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using a epitope-specific immunogen.
Form:Liquid
Synonyms:Anti-Cd81 Antigen antibody annti-26 Kda Cell Surface Protein Tapa-1 antibody, Anti-Target Of The Antiproliferative Antibody 1 antibody, Anti-Tetraspanin-28 antibody, Anti-Tspan-28 antibody, Anti-Cd Antigen Cd81 antibody, Anti-CD81 antibody, Anti-TAPA1 antibody Anti-TSPAN28 antibody.

Applications

Western Blot : 1:500-1:2000 Immunofluorescence: 1:50-200

Immunogen

Synthesized peptide derived from human CD81

Target Background

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This protein appears to promote muscle cell fusion and support myotube maintenance. Also it may be involved in signal transduction. This gene is localized in the tumor-suppressor gene region and thus it is a candidate gene for malignancies. Two transcript variants encoding different isoforms have been found for this gene. Specificity: CD81 polyclonal antibody detects endogenous levels of CD81 protein.

Synonyms:Anti-Cd81 Antigen antibody annti-26 Kda Cell Surface Protein Tapa-1 antibody, Anti-Target Of The Antiproliferative Antibody 1 antibody, Anti-Tetraspanin-28 antibody, Anti-Tspan-28 antibody, Anti-Cd Antigen Cd81 antibody, Anti-CD81 antibody, Anti-TAPA1 antibody Anti-TSPAN28 antibody.

Storage

At +4°C for short term. At -20°C for longer term. Avoid freeze / thaw cycles.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Western blot analysis of lysate from 4T1 cells, using CD81 Antibody.

Western blot analysis of 4T1 cells using CD81 Polyclonal Antibody. Antibody was diluted at 1:2000. Secondary antibody was diluted at 1:20000

Immunofluorescence analysis of mouse-kidney tissue. 1, CD81Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (roomtemperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunofluorescence analysis of rat-spleen tissue. 1, CD81 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

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