Immunological Sciences

CD86 Rabbit Polyclonal Antibody

Jurkat cells were subjected to SDS PAGE followed by western blot with CD86 antibody at dilution of 1:500

Jurkat cells were subjected to SDS PAGE followed by western blot with CD86 antibody at dilution of 1:500

Catalog Number:AB-84793
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:CD86 molecule
Reactivity:Human, Mouse, Rat
Applications:Western Blot: 1:200-1:2000Immunohistochemistry: 1:50-1:500Immunofluorescence: 1:20-1:200
Molecular Weight:75 kDa
Purification:Immunogen affinity purified
Form:Liquid
Synonyms:Activation B7 2 antigen, B7 2, B70, BU63, CD28LG2, CD86, CD86 molecule, CTLA 4 counter receptor B7.2, FUN 1, LAB72
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Applications

Western Blot: 1:200-1:2000Immunohistochemistry: 1:50-1:500Immunofluorescence: 1:20-1:200

Immunogen

CD86 molecule

Target Background

Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. (Microbial infection) Acts as a receptor for adenovirus subgroup B.

Synonyms:Activation B7 2 antigen, B7 2, B70, BU63, CD28LG2, CD86, CD86 molecule, CTLA 4 counter receptor B7.2, FUN 1, LAB72

Storage

Store at -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)

Buffer

PBS with 0.02% sodium azide and 50% glycerol pH 7.3

Jurkat cells were subjected to SDS PAGE followed by western blot with CD86 antibody at dilution of 1:500

Immunofluorescent analysis of RAW264.7 cells using CD86 Antibody at dilution of 1:50.

Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using CD86 Antibody at dilution of 1:200. heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

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