Immunological Sciences

CD9

Western blot analysis of extracts of various cell line, using CD9 antibody at a dilution of 1:1000. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Western blot analysis of extracts of various cell line, using CD9 antibody at a dilution of 1:1000. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Catalog Number:AB-83880
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:Recombinan protein of Human CD9
Reactivity:Hu, Ms, Rt
Applications:Western Blot : 1:1000 Immunohistochemistry: 1:50 - 1:100 Immunofluorescence: 1:50 – 1:100 Immunoprecipitation: 1:20 – 1:50
Molecular Weight:25kDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:CD9; BTCC-1; DRAP-27; MIC3; MRP-1; TSPAN-29; TSPAN29; CD9 antigen

Applications

Western Blot : 1:1000 Immunohistochemistry: 1:50 - 1:100 Immunofluorescence: 1:50 – 1:100 Immunoprecipitation: 1:20 – 1:50

Immunogen

Recombinan protein of Human CD9

Target Background

This gene encodes a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Tetraspanins are cell surface glycoproteins with four transmembrane domains that form multimeric complexes with other cell surface proteins. The encoded protein functions in many cellular processes including differentiation, adhesion, and signal transduction, and expression of this gene plays acritical role in the suppression of cancer cell motility and metastasis.

Synonyms:CD9; BTCC-1; DRAP-27; MIC3; MRP-1; TSPAN-29; TSPAN29; CD9 antigen

Storage

At +4°C for short term. At -20°C for longer term. Avoid freeze / thaw cycles.

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3

Western blot analysis of extracts of various cell line, using CD9 antibody at a dilution of 1:1000. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry of paraffin-embedded human breast carcinoma tissue,using CD9 antibody. The picture on the right is blocked with the synthetized peptide.

Immunofluorescence analysis of HeLa cells using CD9 antibody. The picture on the right is blocked with the synthetized peptide.

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