Immunological Sciences

cGAS Rabbit Monoclonal Antibody

Western blot analysis of extracts of various cell lines, using cGAS antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Western blot analysis of extracts of various cell lines, using cGAS antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Catalog Number:MAB-94713
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:D1D3G
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 400-522 of human cGAS
Reactivity:Hu, Ms, Rt
Applications:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50-1:100 ICC 1:50 - 1:100
Molecular Weight:62KDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:MB21D1;C6orf150;cGAS;h-cGAS;CGAS

Applications

WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50-1:100 ICC 1:50 - 1:100

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 400-522 of human cGAS

Target Background

Nucleotidyltransferase that catalyzes the formation of cyclic GMP-AMP (cGAMP) from ATP and GTP and plays a key role in innate immunity. Catalysis involves both the formation of a 2',5' phosphodiester linkage at the GpA step and the formation of a 3',5' phosphodiester linkage at the ApG step, producing c[G(2',5')pA(3',5')p] . Acts as a key cytosolic DNA sensor, the presence of double-stranded DNA (dsDNA) in the cytoplasm being a danger signal that triggers the immune responses . Binds cytosolic DNA directly, leading to activation and synthesis of cGAMP, a second messenger that binds to and activates TMEM173/STING, thereby triggering type-I interferon production.

Synonyms:MB21D1;C6orf150;cGAS;h-cGAS;CGAS

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.02% sodium azide,50% glycerol,pH7.3.

Western blot analysis of extracts of various cell lines, using cGAS antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Immunohistochemistry of paraffinembedded human colon using Cgas Rabbit MAb at dilution of 1:50 (40x lens)

Immunofluorescence analysis of PC-12 cells using cGAS Rabbit MAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

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