ChAT Rabbit mAb
Western blot analysis of lysates from SH-SY5Y cells, using CHAT Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 5s.
| Catalog Number: | AB19031 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Clone: | ARC0491 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 649-748 of human CHAT (P28329). |
| Reactivity: | Human,Mouse,Rat |
| Applications: | WB,1:1000 - 1:6000 IF/ICC,1:200 - 1:800 ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements. |
| Molecular Weight: | 65kDa |
| Purification: | Affinity purification |
| Form: | liquid |
This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform.
Applications
WB,1:1000 - 1:6000 IF/ICC,1:200 - 1:800 ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 649-748 of human CHAT (P28329).
