CHK1 Mouse Monoclonal Antibody

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Catalog Number:MABN80519
Conjugate:Unconjugated
Size:100μL
Concentration:1mg/ml
Host:Mouse
Isotype:Mouse IgG1
Clone:Monoclonal
Immunogen:Purified recombinant fragment of CHK1 expressed in E. Coli.
Reactivity:Human,Mouse
Applications:WB 1:500-1:2000,ELISA 1:5000-1:20000
Molecular Weight:56kDa
Purification:Affinity Purification
Form:Liquid
Synonyms:CHEK1

Applications

WB 1:500-1:2000,ELISA 1:5000-1:20000

Immunogen

Purified recombinant fragment of CHK1 expressed in E. Coli.

Target Background

CHK1, also known as CHEK1, it is a kinase that phosphorylates cdc25, an important phosphatase in cell cycle control, particularly for entry into mitosis. Cdc25, when phosphorylated on serine 216 by chk1 becomes bound by an adaptor protein in the cytoplasm. Therefore it is inhibited from removing the inhibiting phosphate from MPF (mitotic/maturation promoting factor) added by Wee1. Consequently, a cell is prevented from entering mitosis. Chk1 can also phosphorylate p53 at Ser20 in vitro.

Synonyms:CHEK1

Storage

Store at 4°C short term. Aliquot and store at -20°C for 12 months. Avoid freeze/thaw cycles.

Buffer

Purified antibody in PBS with 0.05% sodium azide.

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