Immunological Sciences

Chromogranin A Rabbit Polyclonal Antibody

Western Blot analysis of various cells using Chr-A Polyclonal Antibody.

Western Blot analysis of various cells using Chr-A Polyclonal Antibody.

Catalog Number:AB-83793
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:The antiserum was produced against synthesized peptide derived from human Chr-A. AA range:311-360
Reactivity:Human,Mouse,Rat
Applications:Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300 Immunofluorescence: 1:50-1:200
Molecular Weight:68kD
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form:Liquid
Synonyms:CHGA; Chromogranin-A; CgA; Pituitary secretory protein I; SP-I

Applications

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300 Immunofluorescence: 1:50-1:200

Immunogen

The antiserum was produced against synthesized peptide derived from human Chr-A. AA range:311-360

Target Background

The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells. This gene product is a precursor to three biologically active peptides; vasostatin, pancreastatin, and parastatin. These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system. Two other peptides, catestatin and chromofungin, have antimicrobial activity and antifungal activity, respectively. Two transcript variants encoding different isoforms have been found for this gene.

Synonyms:CHGA; Chromogranin-A; CgA; Pituitary secretory protein I; SP-I

Storage

Store at -20°C. Avoid repeated freeze-thaw cycles.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Western Blot analysis of various cells using Chr-A Polyclonal Antibody.

Western blot analysis of lysate from A549 cells, using Chr-A antibody.

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Immunohistochemistry analysis of Chr-A antibody in paraffin-embedded human brain tissue.

Immunofluorescent analysis of fixed PC-12 cells at dilution of 1:200.

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