Claudin 11

Western blot analysis of extracts of
various cell lines, using CLDN11
antibody at 1:1000
dilution._Secondary antibody: HRP
Goat Anti-Rabbit IgG (H+L) 
at 1:10000 dilution._Lysates/proteins:
25ug per lane._Blocking buffer: 3%
nonfat dry milk in TBST._Detection:
ECL West Pico Plus 
Exposure time: 90s.

Western blot analysis of extracts of various cell lines, using CLDN11 antibody at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL West Pico Plus Exposure time: 90s.

Catalog Number:AB-84291
Conjugate:Unconjugated
Size:100ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CLDN11.
Reactivity:Hu, Ms, Rt
Applications:Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:100
Molecular Weight:23kDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:CLDN11;OSP;OTM

Applications

Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:100

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CLDN11.

Target Background

This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. The protein encoded by this gene is a major component of central nervous system (CNS) myelin and plays an important role in regulating proliferation and migration of oligodendrocytes. Mouse studies showed that the gene deficiency results in deafness and loss of the Sertoli cell epithelial phenotype in the testis. This protein is a tight junction protein at the human blood-testis barrier (BTB), and the BTB disruption is related to a dysfunction of this gene. Alternatively spliced transcript variants encoding different isoforms have been identified.

Synonyms:CLDN11;OSP;OTM

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.02% sodium azide,50% glycerol,pH7.3.

Western blot analysis of extracts of various cell lines, using CLDN11 antibody at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL West Pico Plus Exposure time: 90s.

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