Immunological Sciences

Cleaved PARP-1 (D214) Rabbit Polyclonal Antibody

Western blot analysis of lysates from A549 cells, treated with etoposide 25uM 24h, using PARP (Cleaved-Asp214) Antibody. The lane on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from A549 cells, treated with etoposide 25uM 24h, using PARP (Cleaved-Asp214) Antibody. The lane on the right is blocked with the synthesized peptide.

Catalog Number:AB-84744
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:The antiserum was produced against synthesized peptide derived from human PARP. AA range:165-214
Reactivity:Human
Applications:Western Blot:   1:500-2000 Immunofluorescence: 1:50-300 Immunohistochemistry: 1:50-300
Molecular Weight:24 kDa
Purification:The antibody was affinity- purified from rabbit antiserum by affinity- chromatography using epitope- specific immunogen.
Form:Liquid
Synonyms:PARP1; ADPRT; PPOL; Poly [ADP-ribose] polymerase 1; PARP- 1; ADP-ribosyltransferase diphtheria toxin-like 1; ARTD1; NAD(+) ADP-ribosyltransferase 1; ADPRT 1; Poly [ADP-ribose] synthase 1

Applications

Western Blot:   1:500-2000 Immunofluorescence: 1:50-300 Immunohistochemistry: 1:50-300

Immunogen

The antiserum was produced against synthesized peptide derived from human PARP. AA range:165-214

Target Background

poly(ADP-ribose) polymerase 1(PARP1) Homo sapiens This gene encodes a chromatin-associated enzyme, poly( ADP- ribosyl) transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.

Synonyms:PARP1; ADPRT; PPOL; Poly [ADP-ribose] polymerase 1; PARP- 1; ADP-ribosyltransferase diphtheria toxin-like 1; ARTD1; NAD(+) ADP-ribosyltransferase 1; ADPRT 1; Poly [ADP-ribose] synthase 1

Storage

Store at -20°C. Avoid repeated freeze-thaw cycles.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Western blot analysis of lysates from A549 cells, treated with etoposide 25uM 24h, using PARP (Cleaved-Asp214) Antibody. The lane on the right is blocked with the synthesized peptide.

Immunofluorescence analysis of Human-stomach-cancer tissue. 1,Cleaved-PARP-1 (D214) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunohistochemical analysis of paraffin-embedded Human-colon-cancer tissue. 1,Cleaved-PARP-1 (D214) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

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