Cleaved-PARP-1 (D214) rabbit Polyclonal Antibody
Western blot analysis of lysates from UV treated: 1) MCF-7, 2) 293T , 3)HELAcells, (Green) primary antibody was diluted at 1:1000, 4°over night, Dylight800 secondary antibody was diluted at 1:10000, 37°1hour. (Red) Actin β Monoclonal Antibody(5B7) (Immunoway:YM3028) antibody was diluted at 1:5000 as loading control, 4° over night, Dylight 680 secondary antibody was diluted at 1:10000, 37° 1hour.
| Catalog Number: | ABE1071 |
| Conjugate: | unconjugated |
| Size: | 100 ug |
| Concentration: | 1 mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Clone: | Polyclonal |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human PARP. AA range:165-214 |
| Reactivity: | Human;Mouse |
| Applications: | WB 1:500-2000, IF 1:50-300, IHC 1:50-300 |
| Molecular Weight: | 24kD |
| Purification: | The antibody was affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form: | liquid |
| Synonyms: | PARP1; ADPRT; PPOL; Poly [ADP-ribose] polymerase 1; PARP-1; ADP-ribosyltransferase diphtheria toxin-like 1; ARTD1; NAD(+) ADP-ribosyltransferase 1; ADPRT 1; Poly[ADP-ribose] synthase 1 |
This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008],
Applications
WB 1:500-2000, IF 1:50-300, IHC 1:50-300
Immunogen
The antiserum was produced against synthesized peptide derived from human PARP. AA range:165-214
