Immunological Sciences

Clock Rabbit Polyclonal Antibody

Western Blot analysis of various cells using CLOCK Polyclonal Antibody with a dilution 1:500 -1:1000

Western Blot analysis of various cells using CLOCK Polyclonal Antibody with a dilution 1:500 -1:1000

Catalog Number:AB-E5789
Conjugate:unconjated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:Polyclonal
Immunogen:The antiserum was produced against synthesized peptide derived from human Clock. AA range:241-290
Reactivity:Human, Mouse, Rat
Applications:Western Blot 1:500- 1:2000 IHC(P) 1:100-1:300
Molecular Weight:95kd
Purification:affinity purified
Form:Liquid
Synonyms:CLOCK; BHLHE8; KIAA0334; Circadia helix-loop-helix protein 8; bHLHe8n locomoter output cycles protein kaput; hCLOCK; Class E basic

Applications

Western Blot 1:500- 1:2000 IHC(P) 1:100-1:300

Immunogen

The antiserum was produced against synthesized peptide derived from human Clock. AA range:241-290

Target Background

The protein encoded by this gene plays a central  role in the regulation of circadian rhythms. The protein encodes a transcription factor of the basic helix-loop-helix (bHLH) family and contains DNA  binding histone acetyltransferase activity. The encoded protein forms a heterodimer with ARNTL (BMAL1) that binds E-box enhancer elements upstream of Period (PER1, PER2, PER3) and Cryptochrome (CRY1, CRY2) genes and activates transcription of these genes. PER and CRY proteins heterodimerize and repress their own transcription by interacting in a feedback loop with CLOCK/ARNTL complexes. Polymorphisms in this gene may  be associated with behavioral changes in certain populations and with obesity and metabolic syndrome.

Synonyms:CLOCK; BHLHE8; KIAA0334; Circadia helix-loop-helix protein 8; bHLHe8n locomoter output cycles protein kaput; hCLOCK; Class E basic

Storage

Store at -20°C. Avoid repeated freeze-thaw cycles.

Buffer

PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide

Western Blot analysis of various cells using CLOCK Polyclonal Antibody with a dilution 1:500 -1:1000

IHC (P) Hu skeletal Muscle tissues using Clock Antibody the picture on the right is blocked with a synthesized peptide

Western Blot of UVEC cell lysate

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