COX4

Western blot analysis of extracts of various cell lines, using COX IV antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L)  at 1:10000 dilution. Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.  Exposure time: 30s.

Western blot analysis of extracts of various cell lines, using COX IV antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 30s.

Catalog Number:MAB- 94626
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Ms
Isotype:IgG1
Clone:6C8
Immunogen:A synthetic peptide of human COX IV
Reactivity:Hu, Ms, Rt, Hm, Mk, Gt
Applications:Western Blot: 1:1000 - 1:4000 Immunohistochemistry: 1:50 - 1:200 Immunocytochemistry: 1:50 - 1:200 Immunoprecipitation: 1:20 - 1:50 Flow cytometry: 1:20 - 1:50
Molecular Weight:17kDa
Purification:Aff. Pur
Form:Liquid
Synonyms:COX4I1;COX IV-1;COX4;COX4-1;COXIV;COXIV-1;COX IV

Applications

Western Blot: 1:1000 - 1:4000 Immunohistochemistry: 1:50 - 1:200 Immunocytochemistry: 1:50 - 1:200 Immunoprecipitation: 1:20 - 1:50 Flow cytometry: 1:20 - 1:50

Immunogen

A synthetic peptide of human COX IV

Target Background

Synonyms:COX4I1;COX IV-1;COX4;COX4-1;COXIV;COXIV-1;COX IV

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.02% sodium azide,50% glycerol,pH7.3.

Western blot analysis of extracts of various cell lines, using COX IV antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 30s.

Immunofluorescence analysis of Hela Cell.1, AF-10 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). COX IV monoclonal antibody (6C8) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 (was diluted at 1:1000 (room temperature, 50 min.). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50 min.).

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