Immunological Sciences

CTGF

Western blot analysis of extracts from various cell lines using CTGF Rabbit pAb (upper) and β-actin (D6A8) Rabbit B Actin lower). Asterisk indicates a 120 kDa band of unknown identity detected by this antibody in Western blot analysis.

Western blot analysis of extracts from various cell lines using CTGF Rabbit pAb (upper) and β-actin (D6A8) Rabbit B Actin lower). Asterisk indicates a 120 kDa band of unknown identity detected by this antibody in Western blot analysis.

Catalog Number:AB-84282
Size:100ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Reactivity:Hu, Ms, Rt
Applications:Western blotting: 1:1000 Immunohistochemistry: 1:100
Molecular Weight:35kDa
Purification:Polyclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val174 of human CTGF protein.
Form:Liquid

Applications

Western blotting: 1:1000 Immunohistochemistry: 1:100

Target Background

Connective tissue growth factor (CTGF, CCN2) belongs to the CCN (CYR61, CTGF, NOV) family of secreted extracellular matrix (ECM) proteins (1). Members of this family contain four conserved cysteine-rich domains, and interact in the ECM with a diverse array of cell surface receptors, includ-ing integrins and heparin-sulfate proteoglycans (2). These interactions regulate a multitude of cellular and tissue functions, including adhesion, proliferation, migration, differentiation, senescence, angiogenesis, inflammation, and wound repair (1, 3-5). The CTGF gene is a transcriptional target of both YAP/TAZ and TGFβ-SMAD signaling pathways (6,7), and aberrant regula-tion of CTGF expression is strongly associated with pathological conditions, notably cancer and fibrosis (8, 9). CTGFRabbit pAb recognizes endogenous levels of total CTGF protein. By Western blot analysis, the antibody detects a 120 kDa protein of unknown identity in both CTGF-positive and CTGF-negative cell lines.

Storage

T -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

Buffer

PBS with 0.02% sodium azide and 50% glycerol pH 7.3

Western blot analysis of extracts from various cell lines using CTGF Rabbit pAb (upper) and β-actin (D6A8) Rabbit B Actin lower). Asterisk indicates a 120 kDa band of unknown identity detected by this antibody in Western blot analysis.

Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using CTGF Antibody at dilution of 1:200 heat mediated antigen retrieved with Tris-EDTA buffer (pH9) .

Mouse heart tissue were subjected to SDS PAGE followed by western blot with CTGF Antibody)at dilution of 1:600

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