CXCR1

Western blot analysis of extracts of various cell lines, using CXCR1
antibody  at 1:1000 dilution.
Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L)at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in
TBST.
Detection: ECL West Pico Plus. 
Exposure time: 15s.

Western blot analysis of extracts of various cell lines, using CXCR1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L)at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 15s.

Catalog Number:AB-82066
Conjugate:Unconjugated
Size:100 μg
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 250-350 of human CXCR1.
Reactivity:Hu
Applications:Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:200
Molecular Weight:70kDa
Purification:Aff.Pur.
Form:Liquid
Synonyms:CXCR1;C-C;C-C-CKR-1;CD128;CD181;CDw128a;CKR-1; CMKAR1;IL8R1;IL8RA;IL8RBA

Applications

Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:200

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 250-350 of human CXCR1.

Target Background

The protein encoded by this gene is a member of the G-protein-coupled receptor family. This protein is a receptor for interleukin 8 (IL8). It binds to IL8 with high affinity, and transduces the signal through a G-protein activated second messenger system. Knockout studies in mice suggested that this protein inhibits embryonic oligodendrocyte precursor migration in developing spinal cord. This gene, IL8RB, a gene encoding another high affinity IL8 receptor, as well as IL8RBP, a pseudogene of IL8RB, form a gene cluster in a region mapped to chromosome 2q33-q36

Synonyms:CXCR1;C-C;C-C-CKR-1;CD128;CD181;CDw128a;CKR-1; CMKAR1;IL8R1;IL8RA;IL8RBA

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.02% sodium azide,50% glycerol,pH7.3.

Western blot analysis of extracts of various cell lines, using CXCR1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L)at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 15s.

Immunohistochemistry of paraffin embedded rat lung using CXCR1 antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin embedded mouse spleen using CXCR1 antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffinembedded human colon using CXCR1 antibody at dilution of 1:100 (40x lens).

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