Cyanine 2, Donkey Anti Rabbit IgG (H+L) Highly cross-ads.

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Catalog Number:IS1040
Size:1 mg
Concentration:2mg/ml
Host:Donkey
Isotype:IgG
Clone:POLY
Reactivity:Rabbit
Applications:Fluorescence microscopy: 1-2 ug/mL FC: 1 ug/106 cells Near-infrared western detection: 50-100 ng/mL
Purification:Aff. Pur.
Form:liquid

Color and Form: yellow solution. Spectral Property: λAbs/λEm = 490/515 nm (in pH 7.4 PBS buffer) CY2 is spectrally similar to DyLight 488, Alexa Fluor 488 and FITC.

Applications

Fluorescence microscopy: 1-2 ug/mL FC: 1 ug/106 cells Near-infrared western detection: 50-100 ng/mL

Target Background

Storage

Store at 4°C for several months. Protect from light. For longer storage, divide the conjugate into small aliquots and freeze at -20°C. Avoid repeated freezing and thawing.

Buffer

pH~7.4 PBS containing 50 mg/mL glycerol 2 mg/mL BSA (IgG-free and protease-free) and 0.0.5% sodium azide.

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