Cyanine 5, Goat Anti Rat IgG (H+L) (min. X Hu, Ms)

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Catalog Number:IS1048
Size:1 mg
Concentration:2mg/ml
Host:Goat
Isotype:IgG
Clone:POLY
Reactivity:Rat
Applications:Recommended Dilution Range Fluorescence microscopy: 1-2 ug/mL Flow cytometry: 1 ug/106 cells Near-infrared western detection: 50-100 ng/m
Purification:Aff. Pur.
Form:liquid

Color and Form: Blue solution. Spectral Properties: λabs/λem = 650/665 nm (in pH 7.4 PBS buffer) CY5 is spectrally similar to Alexa Fluor® 647 and DyLightTM 649

Applications

Recommended Dilution Range Fluorescence microscopy: 1-2 ug/mL Flow cytometry: 1 ug/106 cells Near-infrared western detection: 50-100 ng/m

Target Background

Storage

Store at 4°C for several months. Protect from light. For longer storage, divide the conjugate into small aliquots and freeze at -20°C. Avoid repeated freezing and thawing.

Buffer

2 mg/mL in pH~7.4 PBS containing 50% glycerol, 2 mg/ml bovine serum albumin (IgG-free and protease-free) and 0.05% sodium azide.

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