Cyclin B2 Rabbit Monoclonal Antibody

Western blot detection of Cyclin B2/CCNB2 in Hela,A549,MCF-7,U251 cell lysates using Cyclin B2/CCNB2 antibody(1:1000 diluted).

Western blot detection of Cyclin B2/CCNB2 in Hela,A549,MCF-7,U251 cell lysates using Cyclin B2/CCNB2 antibody(1:1000 diluted).

Catalog Number:MABN87634
Conjugate:Unconjugated
Size:100μL
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Immunogen:Recombinant protein of human Cyclin B2/CCNB2
Reactivity:Human,Mouse,Rat
Applications:WB 1:500-1:2000,IHC 1:50-1:200,ICC/IF 1:200-1:500,IP 1:50-1:100
Molecular Weight:45 kDa
Purification:Affinity purification
Form:liquid
Synonyms:HsT17299

Cyclin B2 is a member of the cyclin family, specifically the B-type cyclins. The B-type cyclins, B1 and B2, associate with p34cdc2 and are essential components of the cell cycle regulatory machinery. B1 and B2 differ in their subcellular localization. Cyclin B1 co-localizes with microtubules, whereas cyclin B2 is primarily associated with the Golgi region. Cyclin B2 also binds to transforming growth factor beta RII and thus cyclin B2/cdc2 may play a key role in transforming growth factor beta-mediated cell cycle control. [provided by RefSeq, Jul 2008]

Applications

WB 1:500-1:2000,IHC 1:50-1:200,ICC/IF 1:200-1:500,IP 1:50-1:100

Immunogen

Recombinant protein of human Cyclin B2/CCNB2

Target Background

Cyclin B2 is a member of the cyclin family, specifically the B-type cyclins. The B-type cyclins, B1 and B2, associate with p34cdc2 and are essential components of the cell cycle regulatory machinery. B1 and B2 differ in their subcellular localization. Cyclin B1 co-localizes with microtubules, whereas cyclin B2 is primarily associated with the Golgi region. Cyclin B2 also binds to transforming growth factor beta RII and thus cyclin B2/cdc2 may play a key role in transforming growth factor beta-mediated cell cycle control. [provided by RefSeq, Jul 2008]

Synonyms:HsT17299

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Buffer

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% protective protein. Stable for 12 months from date of receipt.

Western blot detection of Cyclin B2/CCNB2 in Hela,A549,MCF-7,U251 cell lysates using Cyclin B2/CCNB2 antibody(1:1000 diluted).

Western blot analysis of extracts from HCT116,A431 cells using AMRe87634 at 1:1000.

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