Cytokeratin 20

Western blot analysis of extracts of various cell lines, using Cytokeratin 20 (Cytokeratin 20 (KRT20))
antibody.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

Western blot analysis of extracts of various cell lines, using Cytokeratin 20 (Cytokeratin 20 (KRT20)) antibody. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Catalog Number:AB-80682
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 245-424 of human Cytokeratin 20 (KRT20).
Reactivity:Hu,Ms,Rt
Applications:Western Blot: 1:500 - 1:2000 Immunohistochemistry (paraffin-embedded tissues): 1:50 - 1:100 Immunofluorescence: 1:50 - 1:200 Immunocytochemistry: 1:50 - 1:200
Molecular Weight:48kDa
Purification:Affinity purification
Form:Liquid
Synonyms:K20; CD20; CK20; CK-20; KRT21

Applications

Western Blot: 1:500 - 1:2000 Immunohistochemistry (paraffin-embedded tissues): 1:50 - 1:100 Immunofluorescence: 1:50 - 1:200 Immunocytochemistry: 1:50 - 1:200

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 245-424 of human Cytokeratin 20 (KRT20).

Target Background

The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21.

Synonyms:K20; CD20; CK20; CK-20; KRT21

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.02% sodium azide,50% glycerol,pH7.3.

Western blot analysis of extracts of various cell lines, using Cytokeratin 20 (Cytokeratin 20 (KRT20)) antibody. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry analysis of paraffin-embedded Human colon using Cytokeratin 20 (Cytokeratin 20 (KRT20)) antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunofluorescence analysis of HeLa cells using Cytokeratin 20 (Cytokeratin 20 (KRT20) antibody. Blue: DAPI for nuclear staining.

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