Immunological Sciences

Cytokeratin 8 Rabbit Polyclonal Antibody

Western Blot analysis of various cells using Cytokeratin 8 Polyclonal Antibody diluted at 1:2000

Western Blot analysis of various cells using Cytokeratin 8 Polyclonal Antibody diluted at 1:2000

Catalog Number:AB-83795
Conjugate:Unconjated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:The antiserum was produced against synthesized peptide derived from human Keratin 8. AA range:401-450
Reactivity:Hu, Ms, Rt
Applications:Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000
Molecular Weight:53kDa
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form:Liquid
Synonyms:KRT8; CYK8; Keratin; type II cytoskeletal 8; Cytokeratin-8; CK-8; Keratin-8; K8; Type-II keratin Kb8

Applications

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000

Immunogen

The antiserum was produced against synthesized peptide derived from human Keratin 8. AA range:401-450

Target Background

keratin 8(KRT8) Homo sapiens This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene.

Synonyms:KRT8; CYK8; Keratin; type II cytoskeletal 8; Cytokeratin-8; CK-8; Keratin-8; K8; Type-II keratin Kb8

Storage

Store at -20°C. Avoid repeated freeze-thaw cycles.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Western Blot analysis of various cells using Cytokeratin 8 Polyclonal Antibody diluted at 1:2000

Western Blot analysis of 293 cells using Cytokeratin 8 Polyclonal Antibody diluted at 1:2000

Immunofluorescence analysis of HeLa cells, using Keratin 8 Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using Keratin 8 Antibody. The picture on the right is blocked with the synthesized peptide.

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