Immunological Sciences

DDDDK-Tag Rabbit Polyclonal Antibody

Western Blot analysis using FLAG-tag Polyclonal Antibody against HEK293 cells transfected with vector overexpressing FLAG tag (1) and untransfected (2). Secondary antibody was diluted at 1:20000

Western Blot analysis using FLAG-tag Polyclonal Antibody against HEK293 cells transfected with vector overexpressing FLAG tag (1) and untransfected (2). Secondary antibody was diluted at 1:20000

Catalog Number:AB-84802
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:DDDDK synthetic peptide conjugated to KLH.
Reactivity:Species independent
Applications:Western Blot: 1/1000 - 1/3000 ELISA:1:5000-20000.
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form:Liquid
Synonyms:DDDDK epitope tag; DDDDK epitope tag; DYKDDDDK epitope tag

Applications

Western Blot: 1/1000 - 1/3000 ELISA:1:5000-20000.

Immunogen

DDDDK synthetic peptide conjugated to KLH.

Target Background

The DYKDDDDK (FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal FLAG vectors provide an Ek cleavage site for removal of the fusion tag. The FLAG peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the FLAG peptide is more likely to be accessible to antibodies. A FLAG-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on.

Synonyms:DDDDK epitope tag; DDDDK epitope tag; DYKDDDDK epitope tag

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Western Blot analysis using FLAG-tag Polyclonal Antibody against HEK293 cells transfected with vector overexpressing FLAG tag (1) and untransfected (2). Secondary antibody was diluted at 1:20000

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