Desmin

Western blot analysis of extracts of C2C12 cells, using Desmin antibody at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L)) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECLWest Pico Plus. Exposure time: 30s

Western blot analysis of extracts of C2C12 cells, using Desmin antibody at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L)) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECLWest Pico Plus. Exposure time: 30s

Catalog Number:AB-82627
Conjugate:Unù
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 90-470 of human Desmin.
Reactivity:Hu,Ms,Rt
Applications:Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:100 Immunofluorescence: 1:50 - 1:100 Immunocytochemistry: 1:50 - 1:100
Molecular Weight:55kDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:DES;CSM1;CSM2;LGMD2R;desmin;Desmin

Applications

Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:100 Immunofluorescence: 1:50 - 1:100 Immunocytochemistry: 1:50 - 1:100

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 90-470 of human Desmin.

Target Background

This gene encodes a muscle-specific class III intermediate filament. Homopolymers of this protein form a stable intracytoplasmic filamentous network connecting myofibrils to each other and to the plasma membrane. Mutations in this gene are associated with desmin-related myopathy, a familial cardiac and skeletal myopathy (CSM), and with distal myopathies.

Synonyms:DES;CSM1;CSM2;LGMD2R;desmin;Desmin

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.05% proclin300,50% glycerol,pH7.3.

Western blot analysis of extracts of C2C12 cells, using Desmin antibody at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L)) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECLWest Pico Plus. Exposure time: 30s

Immunohistochemistry of paraffin embedded human colon using Desmin Rabbit pAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol

Immunohistochemistry of paraffin embedded mouse lung using Desmin Rabbit pAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunofluorescence analysis of rat heart using Desmin Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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