DGCR8 Rabbit Polyclonal Antibody

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Catalog Number:ABN00056
Conjugate:Unconjugated
Size:100μL
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:Polyclonal
Immunogen:Recombinant protein of human DGCR8
Reactivity:Human,Mouse,Rat
Applications:WB 1:500-1:1000,ICC/IF 1:50-1:200,IP 1:20-1:50
Molecular Weight:Calculated MW: 86 kDa; Observed MW: 100 kDa
Purification:Affinity Chromatography
Form:Liquid
Synonyms:DGCR8; DGCRK6; Gy1; C22orf12; D16Wis2; pasha

Applications

WB 1:500-1:1000,ICC/IF 1:50-1:200,IP 1:20-1:50

Immunogen

Recombinant protein of human DGCR8

Target Background

Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs.

Synonyms:DGCR8; DGCRK6; Gy1; C22orf12; D16Wis2; pasha

Storage

Store at 4°C short term. Aliquot and store at -20°C for 12 months. Avoid freeze/thaw cycles.

Buffer

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

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