DNA-PKCS

Western blot analysis of extracts of various cell lines, using DNA-PKCS antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.   
Exposure time: 90s.

Western blot analysis of extracts of various cell lines, using DNA-PKCS antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 90s.

Catalog Number:AB-83872
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:A synthetic peptide of human DNA-PKCS
Reactivity:Hu, Ms
Applications:Western Blot: 1:500 -1:2000 Immunohistochemistry: 1:50 - 1:200 Immunofluorescence: 1:50 - 1:200
Molecular Weight:469kDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:DNA-PKCS; DNA-PKcs; DNAPK; DNPK1; HYRC; HYRC1; IM D26; XRCC7; p350; DNA-dependent protein kinase catalytic subunit

Applications

Western Blot: 1:500 -1:2000 Immunohistochemistry: 1:50 - 1:200 Immunofluorescence: 1:50 - 1:200

Immunogen

A synthetic peptide of human DNA-PKCS

Target Background

This gene encodes the catalytic subunit of the DNA-dependent protein kinase (DNAPK). It functions with the Ku70/Ku80 heterodimer protein in DNA double strand break repair and recombination.

Synonyms:DNA-PKCS; DNA-PKcs; DNAPK; DNPK1; HYRC; HYRC1; IM D26; XRCC7; p350; DNA-dependent protein kinase catalytic subunit

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western blot analysis of extracts of various cell lines, using DNA-PKCS antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded human well-differentiated squamous skin carcinoma using DNA-PKCS antibody at dilution of 1:100 (40x lens).

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