DOHH Rabbit Monoclonal Antibody

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Catalog Number:MABN01923
Conjugate:Unconjugated
Size:100μL
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:Monoclonal
Immunogen:A synthetic peptide of human DOHH
Reactivity:Human
Applications:WB 1:500-1:1000,IP 1:20-1:50
Molecular Weight:Calculated MW: 33 kDa; Observed MW: 33 kDa
Purification:Affinity Purification
Form:Liquid
Synonyms:HLRC1; hDOHH

Applications

WB 1:500-1:1000,IP 1:20-1:50

Immunogen

A synthetic peptide of human DOHH

Target Background

Catalyzes the hydroxylation of the N6-(4-aminobutyl)-L-lysine intermediate produced by deoxyhypusine synthase/DHPS on a critical lysine of the eukaryotic translation initiation factor 5A/eIF-5A. This is the second step of the post-translational modification of that lysine into an unusual amino acid residue named hypusine (PubMed:16533814, PubMed:16371467, PubMed:19706422). Hypusination is unique to mature eIF-5A factor and is essential for its function .

Synonyms:HLRC1; hDOHH

Storage

Store at 4°C short term. Aliquot and store at -20°C for 12 months. Avoid freeze/thaw cycles.

Buffer

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

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