Immunological Sciences

DYKDDDK

Fig. 1A Fusion nuclear protein Confocal microscopy of COS-7 cells transfected with expression constructs encoding fusion nuclear protein with DDDDK epitope. 1A - fusion nuclear protein (red) stained with purified anti-DDDDK (F-tag-01) (detection by Goat anti-mouse IgG1 Alexa Fluor® 594)

Fig. 1A Fusion nuclear protein Confocal microscopy of COS-7 cells transfected with expression constructs encoding fusion nuclear protein with DDDDK epitope. 1A - fusion nuclear protein (red) stained with purified anti-DDDDK (F-tag-01) (detection by Goat anti-mouse IgG1 Alexa Fluor® 594)

Catalog Number:MAB-10418
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/mL
Host:Ms
Isotype:IgG1
Clone:F-tag-01
Immunogen:Synthetic peptide: DYKDDDDK conjugated to KLH
Reactivity:All Species
Applications:Immunocytochemistry Western Blotting
Purification:Purified by protein-A affinity chromatography
Form:Liquid

Applications

Immunocytochemistry Western Blotting

Immunogen

Synthetic peptide: DYKDDDDK conjugated to KLH

Target Background

The antibody F-tag-01 recognizes DDDDK-tagged proteins in all species. The small size of this tag and its high hydrophilicity decrease the probability of interference with its expression, proteolytic maturation, antigenicity, localization and function.

Storage

Store at 2-8oC. Do not freeze

Buffer

Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Fig. 1A Fusion nuclear protein Confocal microscopy of COS-7 cells transfected with expression constructs encoding fusion nuclear protein with DDDDK epitope. 1A - fusion nuclear protein (red) stained with purified anti-DDDDK (F-tag-01) (detection by Goat anti-mouse IgG1 Alexa Fluor® 594)

Fig. 1B Cell nuclei 1B - cell nuclei stained with DAPI (blue)

1C - merged figures - confirmation of nuclear localization of the fusion protein; cell nuclei stained with DAPI (blue)

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