E-cadherin Rabbit Polyclonal Antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using E-Cadherin Antibody. The picture on the right is    Blocked with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using E-Cadherin Antibody. The picture on the right is Blocked with the synthesized peptide.

Catalog Number:ABN10278
Conjugate:Unconjugated
Size:100 ug
Concentration:1 mg/ml
Host:Rabbit
Isotype:IgG
Clone:Polyclonal
Immunogen:The antiserum was produced against synthesized peptide derived from human Cadherin. range: 1-50
Reactivity:Human,Mouse,Rat
Applications:WB 1:500-1:1000; IHC 1:100-1:200; ICC/IF 1:100-1:200; ELISA 1:10000-1:20000
Molecular Weight:Calculated MW: 97 kDa
Purification:Affinity purification
Form:Liquid
Synonyms:CDH1; CDHE; UVO; Cadherin-1; CAM120/80; Epithelial cadherin; E-cadherin

Anti-E-cadherin Antibody detects endogenous levels of total E-cadherin protein. CDH1 (cadherin 1) codes for a transmembrane glycoprotein E-cadherin that is located on human chromosome 16q22.1. It is a tumor suppressor gene, which is also known as Cdh1p/Hct1p, Fizzy-related, Ste9, or Srw1.

Applications

WB 1:500-1:1000; IHC 1:100-1:200; ICC/IF 1:100-1:200; ELISA 1:10000-1:20000

Immunogen

The antiserum was produced against synthesized peptide derived from human Cadherin. range: 1-50

Target Background

Anti-E-cadherin Antibody detects endogenous levels of total E-cadherin protein. CDH1 (cadherin 1) codes for a transmembrane glycoprotein E-cadherin that is located on human chromosome 16q22.1. It is a tumor suppressor gene, which is also known as Cdh1p/Hct1p, Fizzy-related, Ste9, or Srw1.

Synonyms:CDH1; CDHE; UVO; Cadherin-1; CAM120/80; Epithelial cadherin; E-cadherin

Storage

Aliquot and store at -20°C. Avoid freeze/thaw cycles.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% protective protein and 0.02% New type preservative N.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using E-Cadherin Antibody. The picture on the right is Blocked with the synthesized peptide.

Western blot analysis of lysates from NIH/3T3 cells, using Cadherin Antibody.The lane of the right is blocked with the synthesized peptide.

Immunofluorescence analysis of rat-lung tissue. 1,E-cadherin Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: mergeofA+B

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