Immunological Sciences

EEA1 (3B7) Rabbit Monoclonal Antibody

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Catalog Number:MABN10306
Conjugate:Unconjugated
Size:100μL
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:Monoclonal
Immunogen:A synthetic peptide of human EEA1
Reactivity:Human,Mouse,Rat
Applications:WB 1:2000-1:20000,ICC/IF 1:200-1:1000
Molecular Weight:162kDa
Purification:Affinity purification
Form:Liquid
Synonyms:Early endosome antigen 1; EEA1; MST105; ZFYVE2;

Applications

WB 1:2000-1:20000,ICC/IF 1:200-1:1000

Immunogen

A synthetic peptide of human EEA1

Target Background

EEA1 is an early endosomal marker and a Rab5 effector protein essential for early endosomal membrane fusion and trafficking (1-2). The carboxy terminus of EEA1 contains a FYVE domain which binds to phosphatidylinositol-3-phosphate (PtdIns(3)P), targeting EEA1 to early endosomes (3). The stable association of EEA1 with the endosomal membrane is regulated by PI3 kinase, Rab5 and calcium/calmodulin (4-6). Once on the membrane, EEA1 interacts with Rab5, NSF and syntaxin 13 to promote early endosomal membrane docking and fusion (7). Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.

Synonyms:Early endosome antigen 1; EEA1; MST105; ZFYVE2;

Storage

Store at 4°C short term. Aliquot and store at -20°C for 12 months. Avoid freeze/thaw cycles.

Buffer

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% New type preservative N and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

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