Immunological Sciences

EGFR / HER1

Western Blot analysis of various cell using EGFR antibody at dilution of 1:2000

Western Blot analysis of various cell using EGFR antibody at dilution of 1:2000

Catalog Number:AB-84336
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:Synthesized peptide derived from human EGFR around the non-phosphorylation site of Y1016.
Reactivity:Hu, Ms, Rt
Applications:Western Blot: 1:500-1:2000 Immunohistochemistry: 1:100-1:300 Immunofluorescence: 1:200-1:1000 ELISA: 1:5000
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using a epitope-specific immunogen.
Form:Liquid

Applications

Western Blot: 1:500-1:2000 Immunohistochemistry: 1:100-1:300 Immunofluorescence: 1:200-1:1000 ELISA: 1:5000

Immunogen

Synthesized peptide derived from human EGFR around the non-phosphorylation site of Y1016.

Target Background

EGFR polyclonal antibody detects endogenous levels of EGFR protein.

Storage

Store at -20°C, and avoid repeat freeze-thaw cycles.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Western Blot analysis of various cell using EGFR antibody at dilution of 1:2000

Immunohistochemical analysis of paraffin-embedded Human- Appendix tissue. 1, EGFR Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1, EGFR Polyclonal Antibody was diluted at 1:200 (4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibodyretrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Immunofluorescence analysis of mouse-liver tissue. 1, EGFR Polyclonal antibody (red) was diluted at 1:200 (+4°C), overnight). 2, Cy3 labeled Secondary antibody was diluted at 1:300 (room temperature, 50min) 3, Picture B: DAPI (Blue) 10 min. Picture A: Target Picture B: DAPI Picture C: merge A+B

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