EHMT2

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Catalog Number:AB-J856
Size:100ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:Synthesized peptide derived from human EHMT2 protein.
Reactivity:Hu, Ms
Applications:Western Blot: 1:500-2000 ELISA: 1:5000-20000
Molecular Weight:133kDa
Purification:EHMT2 antibody was affinity-purified from rabbit antiserum by affinitychromatography using epitope-specific immunogen.
Form:Liquid
Synonyms:Histone-lysine N-methyltransferase EHMT2 Euchromatic histone-lysine Nmethyltransferase 2 HLA-B-associated transcript 8 Histone H3-K9 methyltransferase 3 H3-K9-HMTase 3 Lysine N-methyltransferase 1C Protein G9a

Applications

Western Blot: 1:500-2000 ELISA: 1:5000-20000

Immunogen

Synthesized peptide derived from human EHMT2 protein.

Target Background

Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also mediates monomethylation of 'Lys-56' of histone H3 (H3K56me1) in G1 phase, leading to promote interaction between histone H3 and PCNA and regulating DNA replication. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. May also methylate histone H1. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53. Also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself.

Synonyms:Histone-lysine N-methyltransferase EHMT2 Euchromatic histone-lysine Nmethyltransferase 2 HLA-B-associated transcript 8 Histone H3-K9 methyltransferase 3 H3-K9-HMTase 3 Lysine N-methyltransferase 1C Protein G9a

Storage

Store at -20°C, and avoid repeat freeze-thaw cycles.

Buffer

Liquid form in PBS containing 50% glycerol,and 0.02% sodium azide.

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