Immunological Sciences

EIF2A Rabbit Monoclonal Antibody

Western blot analysis of extracts of various cell lines, using eIF2α antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Western blot analysis of extracts of various cell lines, using eIF2α antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Catalog Number:MAB-94711
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:D7D3
Immunogen:A recombinant protein fragment corresponding to a sequence of human EIF2A
Reactivity:Hu, Ms, Rt
Applications:Western Blot: 1:1000;IF: 1:50; ICC 1:50
Molecular Weight:38kDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:EIF-2;EIF-2A;EIF-2alpha;EIF2;EIF2A;EIF2S1

Applications

Western Blot: 1:1000;IF: 1:50; ICC 1:50

Immunogen

A recombinant protein fragment corresponding to a sequence of human EIF2A

Target Background

The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha

Synonyms:EIF-2;EIF-2A;EIF-2alpha;EIF2;EIF2A;EIF2S1

Storage

Store at -20°C, and avoid repeat freeze-thaw cycles.

Buffer

PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3

Western blot analysis of extracts of various cell lines, using eIF2α antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Western blot analysis of extracts of various cell lines, using eIF2α antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunofluorescence analysis of HeLa using eIF2α Rabbit MAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

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