Immunological Sciences

EP4 Rabbit Polyclonal Antibody

Western Blot analysis of various cells using EP4 Polyclonal Antibody

Western Blot analysis of various cells using EP4 Polyclonal Antibody

Catalog Number:AB-J7155
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:The antiserum was produced against synthesized peptide derived from human PE2R4. AA range:321-370
Reactivity:Human;Rat;Mouse;
Applications:Western Blot: 1/500 - 1/2000 Immunofluorescence:1/200 - 1/1000 ELISA: 1/20000
Molecular Weight:55kDa
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form:Liquid
Synonyms:PTGER4; PTGER2; Prostaglandin E2 receptor EP4 subtype; PGE receptor EP4 subtype; PGE2 receptor EP4 subtype; Prostanoid EP4 receptor

Applications

Western Blot: 1/500 - 1/2000 Immunofluorescence:1/200 - 1/1000 ELISA: 1/20000

Immunogen

The antiserum was produced against synthesized peptide derived from human PE2R4. AA range:321-370

Target Background

The protein encoded by this gene is a member of the G-protein coupled receptor family. This protein is one of four receptors identified for prostaglandin E2 (PGE2). This receptor can activate T-cell factor signaling. It has been shown to mediate PGE2 induced expression of early growth response 1 (EGR1), regulate the level and stability of cyclooxygenase-2 mRNA, and lead to the phosphorylation of glycogen synthase kinase-3. Knockout studies in mice suggest that this receptor may be involved in the neonatal adaptation of circulatory system, osteoporosis, as well as initiation of skin immune responses.

Synonyms:PTGER4; PTGER2; Prostaglandin E2 receptor EP4 subtype; PGE receptor EP4 subtype; PGE2 receptor EP4 subtype; Prostanoid EP4 receptor

Storage

Store at -20°C. Avoid repeated freeze-thaw cycles.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Western Blot analysis of various cells using EP4 Polyclonal Antibody

Western Blot analysis of COLO205 cells using EP4 Polyclonal Antibody

Western blot analysis of lysates from COLO205 cells, using PE2R4 Antibody. The lane on the right is blocked with the synthesized peptide.

Immunofluorescence analysis of MCF7 cells, using PE2R4 Antibody. The picture on the right is blocked with the synthesized peptide.

No documents available.