Immunological Sciences

EPO Rabbit Polyclonal Antibody

Western blot analysis of extracts of various cell lines, using EPO antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 60s.

Western blot analysis of extracts of various cell lines, using EPO antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 60s.

Catalog Number:AB-81525
Conjugate:Unconjugated
Size:100μg
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 100-193 of human EPO
Reactivity:Human, Mouse, Rat
Applications:Western Blot: 1:500 - 1:1000 Immunohistochemistry: 1:100 - 1:200 Immunofluorescence: 1:50 - 1:200 Immunocytochemistry: 1:50 - 1:200
Molecular Weight:21kDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:EPO;EP;MVCD2

Applications

Western Blot: 1:500 - 1:1000 Immunohistochemistry: 1:100 - 1:200 Immunofluorescence: 1:50 - 1:200 Immunocytochemistry: 1:50 - 1:200

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100-193 of human EPO

Target Background

This gene is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types.

Synonyms:EPO;EP;MVCD2

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Western blot analysis of extracts of various cell lines, using EPO antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 60s.

Immunohistochemistry of paraffinembedded human esophagus using EPO antibody (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry of paraffinembedded rat liver using EPO antibody (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry of paraffinembedded human liver damage using EPO antibody (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunofluorescence analysis of mouse liver cells using EPO Rabbit pAb at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

No documents available.