Immunological Sciences

Fibronectin Mouse Monoclonal Antibody

Human plasma tissue were subjected to SDS PAGE followed by western blot with Fibronectin antibody at dilution of 1:6000

Human plasma tissue were subjected to SDS PAGE followed by western blot with Fibronectin antibody at dilution of 1:6000

Catalog Number:MAB-94752
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Mouse
Isotype:IgG1
Clone:3G6
Immunogen:fibronectin 1
Reactivity:Human, Mouse
Applications:Western Blot:  1:1000-1:2000 Immunohistochemistry: 1:  250-1:500 Immunofluorescence:  1:50-1:200
Molecular Weight:Calculated MW: 260 kDa Observed MW: 285kDa
Purification:Protein A+G purification
Form:Liquid
Synonyms:CIG, Cold insoluble globulin, DKFZp686F10164, DKFZp686H0342, DKFZp686I1370, DKFZp686O13149, ED B, Fibronectin, fibronectin 1, FINC, FN, FN1, FNZ, GFND, GFND2, LETS, MSF

Applications

Western Blot:  1:1000-1:2000 Immunohistochemistry: 1:  250-1:500 Immunofluorescence:  1:50-1:200

Immunogen

fibronectin 1

Target Background

Fibronectin 1(FN1) is a high molecular weight glycoprotein which exists in both a soluble form in plasma(plasma FN1) and other body fluids and an insoluble form in the extracellular matrix(cellular FN1). Plasma FN1(dimeric form) is secreted by hepatocytes. Cellular FN(dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. FN1 binds to cell surfaces through integrins and to various compounds including collagen, fibrin and heparin. It is involved in cell adhesion and migration processes including embryogenesis, wound healing, hemostasis, host defense, and metastasis.

Synonyms:CIG, Cold insoluble globulin, DKFZp686F10164, DKFZp686H0342, DKFZp686I1370, DKFZp686O13149, ED B, Fibronectin, fibronectin 1, FINC, FN, FN1, FNZ, GFND, GFND2, LETS, MSF

Storage

Store at -20℃ for 12 months (Avoid repeated freeze / thaw cycles.)

Buffer

PBS with 0.02% sodium azide and 50% glycerol pH 7.4.

Human plasma tissue were subjected to SDS PAGE followed by western blot with Fibronectin antibody at dilution of 1:6000

Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using Fibronectin antibody at dilution of 1:600

Immunoflluorescence Image using Fibronectin antibody

Western Blot analysis of Hela with Fibronectin

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