Immunological Sciences

Firefly Luciferase (7T13) Rabbit Monoclonal Antibody

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Catalog Number:MABN10991
Conjugate:Unconjugated
Size:100μL
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:Monoclonal
Immunogen:A synthetic peptide of Firefly Luciferase
Reactivity:Other
Applications:WB 1:500-1:2000,ICC/IF 1:200-1:500,FC 1:20-1:50
Molecular Weight:61kDa
Purification:Affinity purification
Form:Liquid
Synonyms:Luciferase; Firefly; Luciferin 4 monooxygenase;

Applications

WB 1:500-1:2000,ICC/IF 1:200-1:500,FC 1:20-1:50

Immunogen

A synthetic peptide of Firefly Luciferase

Target Background

Produces green light with a wavelength of 562 nm. Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample. Produces green light with a wavelength of 562 nm.

Synonyms:Luciferase; Firefly; Luciferin 4 monooxygenase;

Storage

Store at 4°C short term. Aliquot and store at -20°C for 12 months. Avoid freeze/thaw cycles.

Buffer

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% New type preservative N and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

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