Fluor® 647 Anti-Mouse Perforin Antibody[S16009A]
C57BL/6 murine splenocytes are stained with FITC Anti-Mouse CD49b Antibody and Fluor<sup>®</sup> 647 Anti-Mouse Perforin Antibody (Left). Splenocytes are stained with FITC Anti-Mouse CD49b Antibody and Fluor<sup>®</sup> 647 Rat IgG2a, κ Isotype Control (Right).
| Catalog Number: | MAB1294UM |
| Conjugate: | Fluor<sup><sup>®</sup></sup> 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 670 nm (e.g., a 660/20 nm bandpass filter). |
| Size: | 100μg |
| Concentration: | 0.5 mg/ml |
| Host: | Rat |
| Isotype: | Rat IgG2a, κ |
| Clone: | S16009A |
| Reactivity: | Mouse |
| Applications: | ICFCM - Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/10<sup>6</sup> cells in 100 μL volume]. |
| Form: | Liquid |
Perforin is a 70 kD cytolytic protein that is expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Perforin is one of the major effector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis.
Applications
ICFCM - Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/10<sup>6</sup> cells in 100 μL volume].