Fluor®700 Anti-Human CD47 Antibody[CC2C6D4]
![Staining of normal human peripheral blood cells with Fluor<sup>®</sup> 700 Anti-Human CD47 Antibody[CC2C6D4] (filled gray histogram) or Fluor<sup>®</sup> 700 Mouse IgG1, κ Isotype Control (empty black histogram). Cells in the lymphocytes gate were used for analysis.](/storage/products/127175/fluor700-anti-human-cd47-antibodycc2c6d4-mab1060m1-img-1.jpg)
Staining of normal human peripheral blood cells with Fluor<sup>®</sup> 700 Anti-Human CD47 Antibody[CC2C6D4] (filled gray histogram) or Fluor<sup>®</sup> 700 Mouse IgG1, κ Isotype Control (empty black histogram). Cells in the lymphocytes gate were used for analysis.
| Catalog Number: | MAB1060M1 |
| Conjugate: | Fluor<sup><sup>®</sup></sup> 700 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 719 nm (e.g., a 725/40 nm bandpass filter). |
| Size: | 100Tests |
| Host: | Mouse |
| Isotype: | Mouse IgG1, κ |
| Clone: | CC2C6D4 |
| Reactivity: | Human |
| Applications: | FCM - Each lot of this antibody is quality control tested by flow cytometric analysis. <b style="color:#0070ca">The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood).</b> Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. |
| Form: | Liquid |
CD47 also known as Rh-associated protein, gp42, integrin-associated protein (IAP), and neurophilin, is a 42-52 kD member of the immunoglobulin superfamily containing a five-pass transmembrane attachment. Two splice variants have been described in the cytoplasmic tail, the shorter form is expressed in bone-marrow-derived cells, endothelial cells, and fibroblasts while the longer form is expressed by neural tissues. CD47 expression is widely distributed in hematopoietic cells including thymocytes, T cells, B cells, monocytes, platelets, and erythrocytes as well as epithelial cells, endothelial cells, fibroblasts, and neural tissues. CD47 functions as an adhesion molecule and thrombospondin receptor and is non-covalently associated with β3 integrins CD51/CD61, CD41/CD61. Thrombospondin is a ligand for CD47; in the absence of CD47 mice show defects in host defense and β3 integrin-dependent ligand binding, migration, and cellular activation. CD47 is also part of the Rh complex on erythrocytes.
Applications
FCM - Each lot of this antibody is quality control tested by flow cytometric analysis. <b style="color:#0070ca">The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood).</b> Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.