FOXO1 Rabbit Polyclonal Antibody

Western blot analysis of various lysates using FOXO1/FOXO3A/FOXO4 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L)  at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 180s.

Western blot analysis of various lysates using FOXO1/FOXO3A/FOXO4 Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 180s.

Catalog Number:AB17978
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Reactivity:Human,Mouse,Rat
Applications:WB 1:500 - 1:2000 IHC-P 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Molecular Weight:80kDa
Purification:Affinity purification
Form:liquid

The Forkhead family of transcription factors mediates many aspects of physiology, including stress response, metabolism, commitment to apoptosis, and development. The Forkhead box subfamily O (FoxO) proteins have garnered particular interest due to their involvement in the modulation of cancer, metabolism and cardiovascular biology.In mammals, four subfamily members have been identified: FOXO1 (previously also known as FKHR), FOXO3 (also known as FKHRL1), FOXO4 (also known as AFX),and FOXO6.

Applications

WB 1:500 - 1:2000 IHC-P 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Immunogen

Synthetic peptide. This information is considered to be commercially sensitive.

Target Background

The Forkhead family of transcription factors mediates many aspects of physiology, including stress response, metabolism, commitment to apoptosis, and development. The Forkhead box subfamily O (FoxO) proteins have garnered particular interest due to their involvement in the modulation of cancer, metabolism and cardiovascular biology.In mammals, four subfamily members have been identified: FOXO1 (previously also known as FKHR), FOXO3 (also known as FKHRL1), FOXO4 (also known as AFX),and FOXO6.

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.01% thimerosal,50% glycerol,pH7.3.

Western blot analysis of various lysates using FOXO1/FOXO3A/FOXO4 Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 180s.

Immunohistochemistry analysis of paraffin-embedded Human placenta using FOXO1/FOXO3A/FOXO4 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat testis using FOXO1/FOXO3A/FOXO4 Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.

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