FOXO1A

Western blot analysis of extracts of
various cell lines, using FOXO1
antibody  at 1:950 dilution.
Secondary antibody: HRP Goat Anti-
Rabbit IgG (H+L)  at 1:10000
dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in
TBST

Western blot analysis of extracts of various cell lines, using FOXO1 antibody at 1:950 dilution. Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST

Catalog Number:AB-82717
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:A synthetic peptide of human FOXO1
Reactivity:Hu, Ms, Rt
Applications:Western Blot: 1:500 - 1:1000 Immunofluorescence: 1:50 - 1:100
Molecular Weight:85kDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:FOXO1; FKH1; FKHR; FOXO1A; forkhead box O1

Applications

Western Blot: 1:500 - 1:1000 Immunofluorescence: 1:50 - 1:100

Immunogen

A synthetic peptide of human FOXO1

Target Background

This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma.

Synonyms:FOXO1; FKH1; FKHR; FOXO1A; forkhead box O1

Storage

Store at -20°C, and avoid repeat freeze-thaw cycles.

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western blot analysis of extracts of various cell lines, using FOXO1 antibody at 1:950 dilution. Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST

Western blot analysis of extracts of various cell lines, using FOXO1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus Exposure time: 90s.

Immunofluorescence analysis of HeLa cells using FOXO1 antibody Blue: DAPI for nuclear staining.

Application: Western blot (WB) Sample 293T Loading Amount 20 ug Dilution1:1000 Incubatition timeover night Temperature 4 ℃ Exposure 15s Observed Band 70 kDa Remarks Western blot analysis of extracts from HEK 293 cells and DLD-1 cells using FOXO1 Antibody (1:1000)

Application Immunofluorescence(IF) Sample SW480 Dilution 1:200 Incubatition time over night Temperature 4 ℃ Remarks Immunofluoresent images of SW480 cells showing nuclear and cytoplasmic localization with FOXO1 Antibody 1:200 (left)

Application: Western blot (WB) Sample HEK293T,DLD-1 Loading Amount25 ug Dilution 1:1000 Incubatition time over night Temperature 4 ℃ Exposure 5s Observed Band 70 kDa Remarks Western blot analysis of extracts of various cell lines, using FOXO1 Polyclonal Antibody.

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