Frataxin

Western blot analysis of extracts of various cell lines, using FXN / Frataxin antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

Western blot analysis of extracts of various cell lines, using FXN / Frataxin antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Catalog Number:AB-83941
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 42-210 of human FXN / Frataxin
Reactivity:Hu, Ms, Rt
Applications:Western Blot: 1:500 -1:1000 Immunohistochemistry: 1:50 - 1:100 Immunofluorescence: 1:50 -1:100
Molecular Weight:14kDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:FXN; CyaY; FA; FARR; FRDA; X25; frataxin

Applications

Western Blot: 1:500 -1:1000 Immunohistochemistry: 1:50 - 1:100 Immunofluorescence: 1:50 -1:100

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 42-210 of human FXN / Frataxin

Target Background

This nuclear gene encodes a mitochondrial protein which belongs to the FRATAXIN family. The protein functions in regulating mitochondrial iron transport and respiration. The expansion of intronic trinucleotide repeat GAA from 8-33 repeats to >90 repeats results in Friedreich ataxia. Alternative splicing results in multiple transcript variants.

Synonyms:FXN; CyaY; FA; FARR; FRDA; X25; frataxin

Storage

Store at -20°C, and avoid repeat freeze-thaw cycles.

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western blot analysis of extracts of various cell lines, using FXN / Frataxin antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry of paraffin-embedded rat heart using FXN / Frataxin antibody at dilution of 1:100 (40x lens).

Immunofluorescence analysis of A549 cells using FXN / Frataxin antibody . Blue: DAPI for nuclear staining

Immunohistochemistry of paraffin-embedded mouse heart using FXN / Frataxin antibody at dilution of 1:100 (40x lens).

No documents available.