Immunological Sciences

GAPDH Monoclonal Antibody(2B8)

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Catalog Number:MABN80003
Size:100μL
Concentration:1mg/ml
Host:Mouse
Immunogen:Synthetic Peptide of GAPDH
Reactivity:human;Rat;Mouse;Mk;Dg;Ch;Hamster;Rabbit;Pig;sheep;Insect;Yeast;Bovine
Applications:WB 1:5000-20000, IHC-P 1:200-300, IF-ICC 1:200
Purification:The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Applications

WB 1:5000-20000, IHC-P 1:200-300, IF-ICC 1:200

Immunogen

Synthetic Peptide of GAPDH

Target Background

Glyceraldehyde-3-phosphate dehydrogenase(GAPDH) Homo sapiens This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, etc.

Storage

Store at 4°C short term. Aliquot and store at -20°C for 12 months. Avoid freeze/thaw cycles.

Buffer

PBS, pH 7.4, containing 0.5%BSA, 0.02% New type preservative N as Preservative and 50% Glycerol.

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