Immunological Sciences

GAPDH Rabbit Polyclonal Antibody

WB analysis of GAPDH. Sample: Protein treated by RIPA Lysis Buffer . Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1: 5000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG , 1: 5000, RT, 1h.

WB analysis of GAPDH. Sample: Protein treated by RIPA Lysis Buffer . Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1: 5000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG , 1: 5000, RT, 1h.

Catalog Number:GB15004-100
Conjugate:unconjugated
Size:100 μL
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Immunogen:KLH conjugated Synthetic peptide corresponding to Mouse GAPDH
Reactivity:Human, Mouse, Rat
Applications:WB 1: 3000-1: 5000 IHC/IF 1: 500-1: 2000 ICC/IF 1: 500-1: 1000
Molecular Weight:36 kDa / 36 kDa
Purification:Affinity purification
Form:Liquid
Synonyms:GAPDH, GAPD, G3PD, HEL-S-162eP, glyceraldehyde-3-phosphate dehydrogenase
Download Datasheet

Applications

WB 1: 3000-1: 5000 IHC/IF 1: 500-1: 2000 ICC/IF 1: 500-1: 1000

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse GAPDH

Target Background

Glyceraldehyde 3-phosphate dehydrogenase (abbreviated as GAPDH or less commonly as G3PDH) is an enzyme of ~37kDa that catalyzes the sixth step of glycolysis and thus serves to break downglucose for energy and carbon molecules. In addition to this long established metabolic function, GAPDH has recently been implicated in several non-metabolic processes, including transcription activation, initiation ofapoptosis,ER to Golgi vesicle shuttling, and fast axonal, or axoplasmic transport.In sperm, a testis-specific isoenzyme GAPDHS is expressed.

Synonyms:GAPDH, GAPD, G3PD, HEL-S-162eP, glyceraldehyde-3-phosphate dehydrogenase

Storage

Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.

Buffer

PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.

WB analysis of GAPDH. Sample: Protein treated by RIPA Lysis Buffer . Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1: 5000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG , 1: 5000, RT, 1h.

WB analysis of GAPDH. Sample: Protein treated by RIPA Lysis Buffer . Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1: 3000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG , 1: 3000, RT, 1h.

IHC analysis of GAPDH. Sample: Human esophageal cancer, 4% PFA 12-24h. Antigen retrieval: Citrate buffer , pressure cooker 2 min. Primary antibody: 1: 1000, 4℃ overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG , 1: 200, RT, 1h.

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