Immunological Sciences

Geminin

Western blot analysis of extracts of various cell lines, using GMNN antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit Exposure time: 90s.

Western blot analysis of extracts of various cell lines, using GMNN antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit Exposure time: 90s.

Catalog Number:AB-81872
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-209 of human GMNN,
Reactivity:Hu
Applications:Western Blot: 1:500 - 1:2000 Immunohistochemistry (paraffin-embedded tissues): 1:50 - 1:200
Molecular Weight:24kDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:GMNN; Gem; MGORS6; geminin

Applications

Western Blot: 1:500 - 1:2000 Immunohistochemistry (paraffin-embedded tissues): 1:50 - 1:200

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-209 of human GMNN,

Target Background

This gene encodes a protein that plays a critical role in cell cycle regulation. The encoded protein inhibits DNA replication by binding to DNA replication factor Cdt1, preventing the incorporation of minichromosome maintenance proteins into the prereplication complex. The encoded protein is expressed during the S and G2 phases of the cell cycle and is degraded by the anaphase-promoting complex during the metaphase-anaphase transition. Increased expression of this gene may play a role in several malignancies including colon, rectal and breast cancer. Alternatively spliced transcript variants have been observed for this gene, and two pseudogenes of this gene are located on the short arm of chromosome 16.

Synonyms:GMNN; Gem; MGORS6; geminin

Storage

Store at -20℃. Avoid freeze / thaw cycles.

Buffer

PBS with 0.02% sodium azide,50% glycerol,pH7.3.

Western blot analysis of extracts of various cell lines, using GMNN antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit Exposure time: 90s.

Immunohistochemistry of paraffinembedded human liver cancer using GMNN antibody at dilution of 1:100 (40x lens).

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